生命学院陈佳教授与合作者受邀发表基因编辑技术系统性综述论文

ON2019-07-07文章来源 生命科学与技术学院CATEGORY新闻

6yue30ri,10fensaicheshengmingkexueyujishuxueyuanchenjiajiaoshouyuzhongkeyuan-mapuxuehuijisuanshengwuxuehuobanyanjiusuoyangliyanjiuyuan(10fensaichetepinjiaoshou),yingyaozaiguojizhimingxueshuqikan《trends in biochemical sciences》shangfabiaotiwei“a tale of two moieties: rapidly evolving crispr/cas-based genome editing”dezongshulunwen,congquanxindejiaoduduicrispr/casjiyinzubianjijishudechuangjianheyingyongjinxingquanmianzongjie,bingduigailingyuweilaikenengdefazhanfangxiangjinxinglezhanwang。

renleijiyinzudnayunhanlerenleishengminghuodongsuobixudeyichuanxinxi,yurenleidejiankangxixixiangguan,erjiyinzudnadeyichang(baokuojianjitubiandeng)zenenggoudaozhirenleijibingdefasheng。liyongjiyinzubianjijishuzekeyitongguojiuzhengjiyinzuzhongdeyichangdnajinerdadaozhiliaorenleiyichuanjibingdemude。tongchangqingkuangxia,jiyinzubianjixitongxuyaolianggezhuyaodegouzaozufenlaishixianjingzhundejiyinzudnabianji,yigeshibiejiyinzudnatedingweizhidedingweizi(locator)heyigeduitedingjiyinzudnajinxingcuihuacaozuodecaozuozi(effector)。liru,zaoqidejiyinzubianjixitongzfnhetalen,fenbieshijiangzfhuotalezuoweilocatoryufoki dnaneiqiemeizuoweieffectorjiehe,keyiduijiyinzudnajinxingcaozuo。

jinnianlai,liyongxijundecrispr/casmianyixitongfazhanqilaidexinyidaijiyinzubianjijishuzaijichukexueheyingyongyanjiufangmiandouqudeleyixiliegemingxingdetupo。crispr/casbenshenjijuyouyujiyinzudnaxiangjiehedelocatorjiegouyu,yejuyouqiegejiyinzudnaxuliedeeffectorjiegouyu,qicaozuojianbian、zhunqueduhebianjixiaolvgao,yincizaishengwuyixueyanjiuzhongbeiguangfanyingyong。gengweizhongyaodeshi,jinxienianlai,yanjiurenyuanjiangcrispr/casjiyinbianjimei(rucrispr/cas9、crispr/cas12a、crispr/cas13deng)yuhesuantuoanbianjimei(rubaomidingtuoanmeiapobec/aid、xianpiaolingtuoanmeitubiantitadahuoadardeng)hefanzhuanlumei(rumoluonishishubaixuebingdufanzhuanlumeimmlv)zhenghefazhanchudejianjibianjixitong(base editor, be)hedaoxiangbianjixitong(prime editor,pe),kezaidanjianjishuipingshixianjingzhungaoxiaodebaxiangxingjiyinbianji。

zaigaizongshulunwenzhong,chenjiajiaoshouheyanglijiaoshouzhuyaoduijiyucrispr/casdexinxingbianjixitongdechuangjianheyingyongjinxinglexiangjindeshuli;jieheqizaijiuzhengrenleijibingxiangguantubiandeyingyongduizheixiexinxingbianjixitongdeyou、quedianjinxinglezongjie;zhenduizheixiebianjixitongqianzaidejiyinzuhezhuanluzutubianjizhijinxingletantao,bingtichuleweilaikenengdejiejuefangan。

wenzhanglianjie:

cas9 nickasezuoweilocatorhehesuantuoanbianjimeihuonizhuanlumeizuoweieffectorxiangjiehegouchengyixiliegaoxiaodejiyinzuxinxingjianjibianjixitong。

10分赛车a,cas9 nickaseyubaomidingtuoanmeijiehejiedaoc-to-tjianjibianji。b,cas9 nickaseyuxianpiaolingtuoanmeitubiantijiehejiedaoa-to-gjianjibianji。c,cas9 nickaseyubaomidingtuoanmeihexianpiaolingtuoanmeitubiantijiehetongshijiedaoc-to-thea-to-gjianjibianji。d,cas9 nickaseyunizhuanlumeijiehejiedaorenyidejianjibianhua、xiaopianduandejianjicharuhuoqueshideng。